| 产品名称: | Influenza B virus |
|---|---|
| 商品货号: | TS152962 |
| Classification: | Orthomyxoviridae, Influenzavirus B |
| Agent: | Influenza B virus |
| Strain: | B/Taiwan/2/62 |
| Applications: | Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format: | frozen 1 mL per vial |
| Storage Conditions: | -70°C or colder |
| Images: | |
| Comments: | Derived by adaptation from allantoic product ATCC® VR-295™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-295™ and ATCC® VR-1735™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture. |
| Effect on Host: | CPE, rounding and degeneration |
| Recommended Host: | MDCK (ATCC® CCL-34x99) |
| Growth Conditions: | Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days |
| Name of Depositor: | RQ Robinson, Centers for Disease Control and Prevention; ATCC® |
| Special Collection: | ATCC |
| Source: | Patient in Taiwan, 1962 |
| References: | Green IJ, et al. The isolation and characterization of a new Influenza Type B virus on Taiwan. Am. J. Hyg. 79: 107-112, 1964. PubMed: 14114349 |