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| 产品名称： | pIIIEx424 tRNA |
|---|---|
| 商品货号： | TS153009 |
| Designations： | pIIIEx424 tRNA |
| Depositors： | DR Engelke |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 7.3000001907348630 DESCRIPTION OF VECTOR: Intact vector size: 7.300 Type of vector: plasmid Cloning sites: EcoRI Polylinker sites: Construction: pRS424 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): TRP1, ->, 468-1142 replicon: f1, ->, 1241-1707 insert detection: lacZ, promoter for in vitro transcription: T7, ->, 1867-1886 restriction site: SalI terminator: RPR1, restriction site: EcoRI promoter: SUP4 tRNA intragenic promoter, restriction site: BamHI promoter for in vitro transcription: T3, promoter: lac, replicon: pMB1 marker(s): ampR, replicon: 2 micron Vector: pIIIEx424 tRNA (plasmid) Promoters: Promoter for in vitro transcription T7 Construction: pRS424 Marker(s):TRP1,ampR Construct size (kb): 7.300000190734863 Features: insert detection: lacZ marker(s): TRP1 marker(s): ampR promoter: SUP4 tRNA intragenic promoter promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: 2 micron replicon: f1 replicon: pMB1 restriction site: BamHI restriction site: EcoRI restriction site: SalI terminator: RPR1 |
| Applications： | YE-type (episomal) shuttle vector vector permitting RNA synthesis in vitro vector permitting expression of small structured RNAs vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.4; BamHI--7.4; HindIII--5.2, 2.1. Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences. One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number. Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS424. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |