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| 产品名称： | pMuTNF 0169 |
|---|---|
| 商品货号： | TS153012 |
| Designations： | pMuTNF 0169 |
| GenBank Number： | M11731 |
| Species： | Mus musculus, mouse |
| Depositors： | B Fennie |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.10099995136261 Tissue: monocyte-macrophage PU5-1.8 cell line (TIB 61) Gene product: tumor necrosis factor, alpha (cachectin) Tnfa |
| Insert Size (kb)： | 1.101 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.6; BglI--2.4, 1.6, 1.05, 0.45; EcoRI--4.5, 1.15; PvuII-- 2.8, 2.5, 0.3; SacI--5.6. The insert contains the following restriction sites (nt from the 5 end): BglI--79, 389; SacI--709; PvuII--752. The insert is the 5 EcoRI fragment from clone lambda6, and contains the 5 untranslated region, the complete coding sequence, and the 3 untranslated region up to the naturally occurring EcoRI site at nt 1105. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Pennica D, et al. Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor. Proc. Natl. Acad. Sci. USA 82: 6060-6064, 1985. PubMed: 3898078 |