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| 产品名称： | pJAK16 JK396 |
|---|---|
| 商品货号： | TS153024 |
| Designations： | pJAK16 JK396 |
| Depositors： | JA Kornacki |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli XL1-Blue |
| Vector Information： | Size (kb): 12.6000003814697300 DESCRIPTION OF VECTOR: Intact vector size: 12.600 Type of vector: plasmid Cloning sites: EcoRI SacI Ecl136II KpnI BamHI XbaI SalI PstI HindIII Polylinker sites: EcoRI SacI Ecl136II KpnI XmaI SmaI BamHI XbaI AccI SalI HincII PstI SphI HindIII Construction: pMMB67EH (ATCC 37622), pSa Host range: broad host range; Escherichia coli Features (with orientation and position when available): promoter: tac MCS: EcoRI...HindIII, -> transcription terminator: rrnB marker(s): cmLR replicon: oriV replicon: oriT replicon: RSF1010 repressor gene: lacIq Vector: pJAK16 (plasmid) Promoters: Promoter tac Construction: pMMB67EH (ATCC 37622), pSa Marker(s):cmlR Construct size (kb): 12.60000038146973 Features: marker(s): cmlR promoter: tac replicon: RSF1010 replicon: oriT replicon: oriV repressor gene: lacIq MCS: EcoRI...HindIII transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--12.6; HindIII--12.6; SphI--9.0, 3.6. A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria. Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids. Can be mobilized by conjugative IncP helper plasmids e.g. pRK2013 (ATCC 37159), pUB307 into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457 Jon A Kornacki, personal communication |
| Related Products： | component of:ATCC 87055 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |