微信小陈
微信小章| 产品名称： | pHBI59 |
|---|---|
| 商品货号： | TS153041 |
| Designations： | pHBI59 |
| Species： | Homo sapiens, human |
| Applications： | Mapped to the pericentromeric region by multipoint linkage analysis. This probe has been used to detect loss of heterozygosity at MEN1. Some tumors retain this sequence. Also detects a TaqI polymorphism not yet verified by pedigree analysis. |
| Vector： | Construct size (kb): 4.289999961853027 |
| Insert： | DNA: genomic Insert lengths(kb): 1.600000023841858 Gene product: DNA Segment, single copy D11S146 Alleles: A1, B1, B2, A2 |
| Insert Size (kb)： | 1.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.97, 0.52. An EcoRI/HindIII double digest shows insert fragment sizes of 0.9 and 0.4 kb. Mapped to the pericentromeric region by multipoint linkage analysis. This probe has been used to detect loss of heterozygosity at MEN1. Some tumors retain this sequence. MEN1 maps between PYGM and D11S146. Detects human genomic restriction fragments of the following sizes (kb): BssHI--600; ClaI--850, 700, 600, 450; NruI--1500; SacII--600; SalI--950, 800, 600. Codominant segregation of the MspI polymorphism has been demonstrated in 50 3-generation families. Also detects a TaqI polymorphism not yet verified by pedigree analysis. Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII, RsaI. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pHBI59) on chromosome 11 D11S146. Nucleic Acids Res. 16: 10404, 1988. PubMed: 2461547 Nakamura Y, et al. Localization of the genetic defect in multiple endocrine neoplasia type I within a small region of chromosome 11. Am. J. Hum. Genet. 44: 751-755, 1989. PubMed: 2565085 Szepetowski P, et al. D11S146 and BCL1 are physically linked but can be discriminated by their amplification status in human breast cancer. Genomics 10: 410-416, 1991. PubMed: 2071147 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pHBI59 on chromosome 11 D11S146. Nucleic Acids Res. 16: 376, 1988. PubMed: 2448753 Yusuke Nakamura, personal communication |