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购买须知
| 产品名称： | pSC161RM O/P17 |
|---|---|
| 商品货号： | TS153044 |
| Designations： | pSC161RM O/P17 |
| Species： | Acinetobacter calcoaceticus (Beijerinck) Baumann et al. |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | in suitable host, produces protein modification methylase AccI in suitable host, produces protein restriction endonuclease AccI |
| Insert： | DNA: genomic Insert lengths(kb): 4.199999809265137 Gene product: restriction endonuclease AccI hsdM Target Gene: modification methylase AccI, restriction endonuclease AccI |
| Insert Size (kb)： | 4.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI/PstI--4.5, 4.0, 2.6. The ClaI fragment (which includes pBR322 ClaI/EcoRI sequence) contains the following restriction sites (approximate kb from the vector-derived end): EcoRI--0.02, 1.8; XmnI--0.9, 3.9; HindIII--3.3, 3.5; BanII--4.1; KpnI--4.1. To optimize production, the plasmid should be transformed into E. coli MM294, grown at 30C to late log phase, and then shifted to 42C for an additional 3 hr. The vector is pUC19 modified by insertion of a 4.3 kb BamHI fragment containing bacteriophage lambda N, PR, cI857, and PR sequences. The PL promoter drives transcription of the insert. Distributed in aliquots of 100 ng dried. |
| References： | Chen S, Wilson GG. Method for producing the AccI restriction endonuclease and methylase. US Patent 5,004,691 dated Apr 2 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |