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| 产品名称： | pBP81 |
|---|---|
| 商品货号： | TS153061 |
| Designations： | pBP81 |
| Depositors： | RH Reeves |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.1999998092651370 Vector: pBP81 (plasmid) Construction: pBP63A, YTCA-1x, CEN6 Marker(s):ampR,HIS3 Construct size (kb): 6.199999809265137 Features: marker(s): ampR, HIS3 replicon: pMB1 centromere: CEN6 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.35, 0.25; PstI--4.4, 1.4, 0.4; SstI--5.4, 0.8; SalI/SphI--6.2. Linearize with SalI (site adjacent to the telomere sequence) before transformation. Centric chromosome fragmentation vector targeting Alu sequences. Constructed by replacing the telomere-adjacent sequence from pBP63A with a 0.25 kb SalI/SphI fragment containing the YTCA-1x telomere and CEN6 sequences. Contains 2 head to tail Alu sequences with the 5 end closer to the SalI site. The order of the major features in this plasmid is: bla - HIS3 - 3Alu5 - 3Alu5 - SalI - TEL - CEN6. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Pavan WJ, et al. High-efficiency yeast artificial chromosome fragmentation vectors. Gene 106: 125-127, 1991. PubMed: 1937033 Reeves RH, et al. Yeast artificial chromosome modification and manipulation. Methods Enzymol. 216: 584-603, 1992. PubMed: 1336105 |
| Shipped： | freeze-dried |