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| 产品名称： | pAB366 pAmPh366 |
|---|---|
| 商品货号： | TS153140 |
| Designations： | pAB366 pAmPh366 |
| Depositors： | BM Tyler |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.1999998092651370 Vector: pAB366 (plasmid) Construction: pRMH200, pAm200 Marker(s):ampR bleR Construct size (kb): 4.199999809265137 Features: marker(s): ampR bleR replicon: pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.7, 1.5; BamHI/XhoI--3.4, 0.8; BglI--3.0, 1.2. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. Shuttle vector conferring bleomycin and phleomycin resistance. The order of the major features in this plasmid is: pUC8 - EcoRI - BamHI - am promoter - ble - XhoI - am 3 region - HindIII - pUC8. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844 |
| Shipped： | freeze-dried |