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| 产品名称： | pCyR-1 PL-1021 |
|---|---|
| 商品货号： | TS153184 |
| Designations： | pCyR-1 PL-1021 |
| Species： | Cymbidium ringspot virus |
| Depositors： | DSM |
| Vector： | Construct size (kb): 6.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.799999952316284 Gene product: coat protein; 22K protein |
| Insert Size (kb)： | 3.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.9; BamHI/HindIII--3.2, 2.7, 0.6; PstI--3.8, 2.7; HindIII--6.5; BamHI--3.5, 3.0. The insert includes the following sites (approximate kb from the 5 end of the insert): BamHI--0.27; EcoRI--1.1; ApaI--1.6; BglII--2.2; KpnI--2.4. Encodes about 80% of the virus genome from the 3 end. Hybridizes to three RNAs from infected plant tissue: a genomic RNA (about 4.7 kb), a 2.1 kb RNA and a 1.0 kb RNA. |
| Classification： | Tombusvirus |
| References： | Russo M, et al. Complementary DNA cloning and characterization of cymbidium ringspot virus RNA. J. Gen. Virol. 69: 401-406, 1988. |