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| 产品名称： | Influenza B virus |
|---|---|
| 商品货号： | TS153191 |
| Classification： | Orthomyxoviridae, Influenzavirus B |
| Agent： | Influenza B virus |
| Strain： | B/Lee/40 |
| Applications： | Influenza Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of egg allantoic fluid product ATCC VR-101 to MDCK cells (ATCC CCL-34). ATCC VR-101 and TS153191 have not been compared for sequence or infectivity in eggs and tissue culture. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | MDCK (ATCC® CCL-34™) |
| Growth Conditions： | Temperature: 37°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
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| Name of Depositor： | MT Coleman, Centers for Disease Control and Prevention |
| Special Collection： | NCRR Contract |
| Source： | Throat washings from an influenza patient, Irvington, New York, 1940 |
| References： | Andrewes CH, et al. A short description of the Myxovirus group (Influenza and related viruses). Virology 1: 176-184, 1955. PubMed: 13267985 Francis T Jr. A new type of virus from epidemic influenza. Science 92: 405-408, 1940. PubMed: 17794275 |