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| 产品名称： | pYAC4 |
|---|---|
| 商品货号： | TS153249 |
| Designations： | pYAC4 |
| Depositors： | MV Olson |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.4540004730224600
Vector: pYAC4 (YAC)
Marker(s): TRP1,URA3,ampR,HIS3,SUP4
Construct size (kb): 11.454
Features: marker(s): HIS3 marker(s): SUP4 marker(s): TRP1 marker(s): URA3 marker(s): ampR other: telomere replicon: ARS1 replicon: pMB1 centromere: CEN4 |
| Applications： | YL-type (YAC) shuttle vector Shuttle vector Vector for constructing genomic libraries |
| Comments： | Yeast artificial chromosome (YAC) vector for cloning EcoRI fragments
A 0.7 kb BamHI-XhoI fragment contains two copies of the Tetrahymena thermophila macronuclear telomere
This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825 Wells RA, et al. Telomere-related sequences at interstitial sites in the human genome. Genomics 8: 699-704, 1990. PubMed: 2276741 Burke DT, Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194: 251-270, 1991. PubMed: 2005791 Kuhn RM, Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene 141: 125-127, 1994. PubMed: 8163163 |
| Shipped： | frozen |