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| 产品名称： | lambda92 GC.92 |
|---|---|
| 商品货号： | TS153298 |
| Designations： | lambda92 GC.92 |
| GenBank Number： | L10641 |
| Species： | Homo sapiens, human |
| Depositors： | A Dugaiczyk |
| Vector： | Construct size (kb): 48.79999923706055 DESCRIPTION OF VECTOR COMPONENT: Name of vector: Charon 4A Intact vector size: 46.000 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none promoter: none replicon: lambda terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GC Genomic copy number: unique Gene name: group-specific component (vitamin D binding protein) Contains complete coding sequence?: N Chromosome: 4; Localization: 4 q12-q13 Tissue: fetal liver (ATCC 37333) Type of DNA: genomic Insert end: HaeIII or AluI Modification: EcoRI linker Insert end: HaeIII or AluI Modification: EcoRI linker Insert size (kb): 17.85 Cross references: DNA Seq. Acc.: L10641 Nucleotides ~1-17850 of the insert correspond to nucleotides ~19800-37650 of L10641. Insert lengths(kb): 17.85000038146973 Tissue: fetal liver (ATCC 37333) Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) GC |
| Insert Size (kb)： | 17.850 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 6.2, 3.5, 2.7, 2.5, 1.9, 1.15; HindIII--18 (doublet), 6.2, 2.4, 1.4, 1.1, 1, 0.4; BamHI--14 (doublet), 8, 6.2, 4, 1.5, 0.5; SstI-->23, 8.5, 4.2; BglII--18, 5.2, 4.85, 4.7, 3.7, 2.2, 2.1, 1.9, 1.4, 1.0, 0.9, 0.8, 0.75, 0.65, 0.4, 0.3. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.9, 1.05, 2.95, 5.45, 11.65, 14.35; SacI--2.4; HindIII--8.1, 10.4, 11.5, 12.8, 13.8, 14.2. Begins in intron 2 and extends into intron 11. ATCC 95664/95665 and 95666/95667 overlap by 6.45 kb and share EcoRI fragments of the following sizes (kb): 0.15, 1.9, 2.5. TS153298/95667 and 95668/95669 overlap by 7.8 kb and share an EcoRI fragment of 2.7 kb. Contains Alu repeats, Kpn repeats, and a THE repeat. |
| References： | Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650 |