| 产品名称: | pI7-11 |
|---|---|
| 商品货号: | TS153305 |
| Designations: | pI7-11 |
| GenBank Number: | M73047 |
| Species: | Homo sapiens, human |
| Depositors: | B Tomkinson |
| Vector: | Construct size (kb): 4.841000080108643 |
| Insert: | DNA: cDNA Insert lengths(kb): 2.141000032424927 Tissue: B lymphocyte (EBV transformed) Gene product: tripeptidylpeptidase 2 TPP2 |
| Insert Size (kb): | 2.141 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--2.75, 2.15; SalI--2.9, 2.05; PstI--4.3, 0.47, 0.32; PvuII--2.4, 1.05, 0.90, 0.64; HindIII--4.9. The insert contains the following restriction sites (approximate kb from the 5 end): SalI--0.16; SmaI--0.17; PvuII--0.74, 1.35; PstI--1.48, 1.92. Corresponds to nt 1-2141 of the published sequence, encoding the N-terminus of the protein. One of 4 clones (pI7-11, pF5-1, pA2-10, pA2-13, TS153305-65995) that together contain the complete coding sequence. Cloned in the antisense direction. |
| References: | Tomkinson B. Nucleotide sequence of cDNA covering the N-terminus of human tripeptidyl peptidase II. Biomed. Biochim. Acta 50: 727-729, 1991. PubMed: 1840501 Birgitta Tomkinson, personal communication |