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微信小章| 产品名称： | zif/268 |
|---|---|
| 商品货号： | TS153328 |
| Designations： | zif/268 |
| GenBank Number： | J04089 |
| Species： | Mus musculus, mouse |
| Depositors： | D Nathans |
| Applications： | can be used to produce riboprobes early growth response 1 krox-24, zif-268 |
| Vector： | Construct size (kb): 6.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.200000047683716 Tissue: activated 3T3 cell line Gene product: early growth response 1(krox-24, zif-268) Egr1 Target Gene: early growth response 1 |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Among the restriction sites in the insert are the following (in bp from the T7 promoter side 5): Pst--317; BglII--321;, NdeI--1273; BglII--1963; PstI--2905. Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 3.0; HindIII--6.2; PstI--3.2, 2.7, 0.35; BglII--4.7, 1.7; BamHI--6.2. zif268 is induced in mouse 3T3 cells by serum, tetradecanoylphorbol acetate, PDGF, and FGF. Induction is coordinate with c-fos. Genomic 5 flanking regions contain multiple serum response elements (SRE) which compete with the c-fos SRE. Encodes a protein of 533 amino acids with three typical zinc finger sequences. Between the polyadenylation signals at the 3 end of the message are two ATTTA sequences, related to the message instability characteristics of immediate early mRNAs. Homologous to NGFI-A, which is activated in rat pheochromocytoma cells by nerve growth factor. Within the zinc finger region it is related to the Krox gene family (Krox24). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Christy BA, et al. A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences. Proc. Natl. Acad. Sci. USA 85: 7857-7861, 1988. PubMed: 3141919 Christy B, Nathans D. Functional serum response elements upstream of the growth-factor inducible gene zif268. Mol. Cell. Biol. 9: 4889-4895, 1989. PubMed: 2513479 Chaudhuri A, et al. Dual activity maps in primate visual cortex produced by different temporal patterns of zif268 mRNA and protein expression. Proc. Natl. Acad. Sci. USA 94: 2671-2675, 1997. PubMed: 9122254 Daniel Nathans, personal communication |