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微信小章| 产品名称： | 28-5-15 |
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| 商品货号： | TS153393 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC. The antibody binds the VH1/V kappa 24 heavy and light chain idiotypic specificities of the phosphocholine specific myeloma protein, MOPC167. This antibody can be used as a typing reagent for mouse Ig bearing the VH1/V kappa 24 idiotypic specificity and can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of MOPC167 myeloma. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against MOPC167 idiotype (VH1/V kappa 24) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against MOPC167 idiotype (VH1/V kappa 24) |
| Comments： | Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody binds the VH1/V kappa 24 heavy and light chain idiotypic specificities of the phosphocholine specific myeloma protein, MOPC167. It completely inhibits the binding of MOPC167 to phosphocholine. It can bind the membrane receptor of 207-4 transgenic B cells and induce proliferation of these B cells. The antibody does not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins. This antibody can be used as a typing reagent for mouse Ig bearing the VH1/V kappa 24 idiotypic specificity and can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of MOPC167 myeloma. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 viable cells/mL.xa0 Maintain cultures at a cell concentration between 5xa0 x 104 andxa0 5 x 105 cells/mL. Do not allow the cell concentration to exceed 5 x 105 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | rat IgG2a lambda |
| Name of Depositor： | DG Sieckmann |
| Deposited As： | rat (B cell); mouse (myeloma) |
| Year of Origin： | 1990 |
| References： | Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |