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| 产品名称： | pRB382 |
|---|---|
| 商品货号： | TS153421 |
| Designations： | pRB382 |
| Depositors： | R Bruckner |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.900
Vector: pRB382 (plasmid)
Promoters: Promoter vegII (B. subtilis)
Construction: pUB110, pBR322, lacZ, vegII
Marker(s): ampR,bleR,neoR
Construct size (kb): 8.900
Features: marker(s): ampR marker(s): bleR marker(s): neoR promoter: vegII (B. subtilis) replicon: pMB1 replicon: pUB110 reporter group: lacZ MCS: HindIII...BamHI terminator: To phage lambda transcription terminator: rrnB |
| Applications： | expression vector shuttle vector vector permitting visual detection of activity in colonies |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.5; EcoRI/BglI--5.1, 2.2, 1.1, 0.8; BglI/BglII--3.5, 2.2, 2.0, 1.4. The Bacillus subtilis promoter vegII initiates transcription in both B. subtilis and E. coli. Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. May not be suitable for cloning very strong expression signals. Translational fusion shuttle vector using lacZ as reporter. Also contains the vegII promoter. Useful for construction of fusion proteins. Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. The 5 truncated lacZ gene, lacking transcriptional and translational initiation signals, is useful in both E. coli and in B. subtilis, since the endogenous beta-galactosidase level in B. subtilis is low. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028 |
| Related Products： | Component of: ATCC 87056 |
| Shipped： | frozen |