微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pUC-Cathepsin G pUC-Cathepsin |
|---|---|
| 商品货号： | TS153479 |
| Designations： | pUC-Cathepsin G pUC-Cathepsin |
| GenBank Number： | J04990 |
| Species： | Homo sapiens, human |
| Depositors： | TJ Ley |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Tissue: lung fibroblast Gene product: cathepsin G CTSG |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | This insert includes an internal HindIII site which maps to exon 5. Restriction digests of the clone give the following sizes (kb): BamHI--5.7; HindIII--3.0, 2.7; EcoRI--3.0, 2.7; HincII--5.7. A unique probe for cathepsin G can be made by purifying the 0.35 kb HindIII/EcoRI fragment derived from exon 5 and the 3 flanking region of this gene. The insert is an EcoRI fragment that begins at position -76 with respect to the transcription initiation site and ends about 300 bp downstream of the poly(A) site. The cathepsin G gene is divided into 5 exons and 4 introns, similar to several other serine proteases, including human neutrophilelastase, rat mast cell protease II, murine adipsin and murine and human cytotoxic T-cell serine proteases. At high stringency, this probe hybridizes to a genomic BamHI fragment of 6.0 kb, a genomic BglII fragment of 8.5 kb, and a genomic EcoRI fragment of about 3.0 kb. There is also a polymorphic 4.3 kb BglII fragment. |
| References： | Hohn PA, et al. Genomic organization and chromosomal localization of the human cathepsin G gene. J. Biol. Chem. 264: 13412-13419, 1989. PubMed: 2569462 Hanson RD, et al. Developmental regulation of the human cathepsin G gene in myelomonocytic cells. J. Biol. Chem. 265: 1524-1530, 1990. PubMed: 2295643 Timothy J Ley, personal communication |