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| 产品名称： | pC194Amy |
|---|---|
| 商品货号： | TS153493 |
| Designations： | pC194Amy |
| Species： | Geobacillus stearothermophilus (Donk) Nazina et al. |
| Depositors： | CPC International, Inc. |
| Applications： | The genotype for the host strain is phe tyr trp sacA. |
| Vector： | Construct size (kb): 8.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pC194 Intact vector size: 2.900 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: HaeIII HindIII BglI Polylinker sites: Construction: natural isolate Host range: Bacillus sp.; Staphylococcus aureus Features (with orientation and position when available): marker(s): cmlR replicon: pC194 Cross references: DNA Seq. Acc.: L08860 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pC194 Intact vector size: 2.900 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: HaeIII HindIII BglI Polylinker sites: Construction: natural isolate Host range: Bacillus sp.; Staphylococcus aureus Features (with orientation and position when available): marker(s): cmlR replicon: pC194 Cross references: DNA Seq. Acc.: L08860 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus stearothermophilus Gene name: alpha-amylase Contains complete coding sequence?: U Strain: N21 Type of DNA: genomic Insert end: HindIII Insert end: HindIII Insert size (kb): 3.0 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus stearothermophilus Gene name: alpha-amylase Contains complete coding sequence?: U Strain: N21 Type of DNA: genomic Insert end: HindIII Insert end: HindIII Insert size (kb): 3.0 Cross references: Insert lengths(kb): 3.0 Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A) |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The genotype for the host strain is phe tyr trp sacA. Plasmid encodes a thermostable alpha-amylase. The probe was constructed from pC194 and Bacillus stearothermophilus plasmid DNA. |
| References： | Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |