微信小陈
微信小章| 产品名称： | Plasmodium falciparum Welch |
|---|---|
| 商品货号： | TS153552 |
| Strain Designations： | Honduras |
| Application： | Emerging infectious disease research Vector borne research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | human, Honduras |
| Product Format： | frozen |
| Type Strain： | no |
| Growth Conditions： | Duration: in vivo, Aotus trivirgatus Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus). |
| Subcultivation： | Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus). |
| Cryopreservation： | CRYOPRESERVATION:xa0 Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust. 1.xa0xa0xa0xa0 To harvest parasites, inject host with ketamine (0.5x961.0ml depending on host size / weight). 2.xa0xa0xa0xa0 Exsanguinate via the femoral vein using a sterile syringe and Yaegers anticoagulant**, 1 volume anticoagulant to 4 volumes blood.xa0 Note: Unless there is an exchange transfusion, no more than 10% of estimated total blood volume should be collected over a 2 wk. period. 3.xa0xa0xa0xa0 Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube. 4.xa0xa0xa0xa0 Aspirate supernatant using sterile Pasteur pipet. 5.xa0xa0xa0xa0 Resuspend pellet gently in remaining supernatant. 6.xa0xa0xa0xa0 Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows: xa0xa0xa0xa0xa0xa0xa0 A.xa0xa0xa0 Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature. B.xa0xa0xa0 Add the remaining 4 volumes of glycerolyte and gently agitate. 7.xa0xa0 Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.). 8.xa0xa0xa0xa0 Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor. xa0xa0xa0xa0xa0xa0xa0 |
| Name of Depositor： | National Institutes of Health |
| Chain of Custody： | ATCC < |