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| 产品名称： | pMLSV N1/N4-S |
|---|---|
| 商品货号： | TS153558 |
| Designations： | pMLSV N1/N4-S |
| GenBank Number： | X01057 |
| Species： | Homo sapiens, human |
| Depositors： | Immunex Corporation, S Gillis, Immunex Corporation |
| Applications： | in another host, produces protein interleukin 2 receptor, alpha IL-2R alpha chain, Interleukin-2 receptor, IL-2 receptor |
| Vector： | Construct size (kb): 5.670000076293945 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pMLSV Intact vector size: Type of vector: plasmid Vector end: PstI Vector end: BglII Cloning sites: BglII PstI Polylinker sites: BglII PstI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 early replicon: pMB1, SV40 ori terminator: SV40 polyadenylation Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IL2RA Other gene symbols: IL2R Genomic copy number: unique Gene name: interleukin 2 receptor, alpha Contains complete coding sequence?: Y Chromosome: 10; Localization: 10 p15-p14 Tissue: lymphoma, cutaneous T cell, HUT 102 cell line Type of DNA: cDNA Insert 5 end: PstI Insert 3 end: BamHI Insert size (kb): 1.79 Cross references: DNA Seq. Acc.: X01057 Nucleotides 1-1790 of the insert correspond to nucleotides 132-1921 of X01057. Insert lengths(kb): 1.789999961853027 Tissue: lymphoma, cutaneous T cell, HUT 102 cell line Gene product: interleukin 2 receptor, alpha( IL-2R alpha chain, Interleukin-2 receptor, IL-2 receptor) IL2R Target Gene: interleukin 2 receptor, alpha |
| Insert Size (kb)： | 1.790 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/SstI--2.6, 2.5, 0.5; PstI--3.0, 2.2, 0.4; XbaI--4.7, 0.9; BamHI/PstI--2.3, 2.0, 0.9, 0.42. Expresses a functional IL-2 receptor in COS-7 cells. The insert contains the following restriction sites (approximate kb from the 5 end): SacI--0.12; PstI--0.43; XbaI--0.87. A SacI/BamHI fragment detects transcripts of 1.4 and 3.4 kb in activated T cells and HUT 102 cells. |
| References： | Cosman D, et al. Cloning, sequence, and expression of human interleukin-2 receptor. Nature 312: 768-771, 1984. PubMed: 6096720 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |