微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pPLc28 PL-C, pST28 |
|---|---|
| 商品货号： | TS153562 |
| Designations： | pPLc28 PL-C, pST28 |
| Depositors： | Biogen, Inc., JF Haley, Biogen, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli deltaH1; K-12 delta H1 Distribution host: Escherichia coli deltaH1; K-12 delta H1 |
| Vector Information： | Size (kb): 2.7200000286102300 Vector: pPLc28 (plasmid) Promoters: Promoter lambda PL Construction: pBR322, lambda Marker(s):ampR Construct size (kb): 2.72000002861023 Features: marker(s): ampR promoter: lambda PL replicon: pMB1 enhancer: none |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; BamHI--2.7; HindIII--2.7. The following unique restriction sites are found on this vector separated by (bp)(approx): BglI- 100- PstI- 150- PvuI- 1020- EcoRI- 10- BamHI-HindIII-1420. Plates equally well at 28C and 42C in E. coli K-12 deltaH1 hosts. Escherichia coli K-12 deltaH1 is K-12 M72 lac(am) deltatrpEA2 rpsL lambda cI857 N(am7)N(am53) deltaH1 bio-. deltaH1 removes part of cro and all genes to the right of cro. Shows reduced plating efficiency in E. coli M5219 at 42C under antibiotic selection. This vector is used for expression of fragments containing a ribosome binding site. The orientation of the PL promoter is clockwise with respect to the plasmid ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 28.0°C |
| References： | Fiers WC, Remaut ER. Vectors and methods for making such vectors and for expressing cloned genes. US Patent 4,874,702 dated Oct 17 1989 Remaut E, et al. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene 15: 81-93, 1981. PubMed: 6271633 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |