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| 产品名称： | pDdGal-17 |
|---|---|
| 商品货号： | TS153596 |
| Designations： | pDdGal-17 |
| Depositors： | AJ Harwood |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.5000000000000000 Vector: pDdGal-17 (plasmid) Promoters: Promoter none Construction: pDdlac-1, pB10TP2 Marker(s):ampR Construct size (kb): 8.5 Features: insert detection: lacZ marker(s): ampR promoter: none replicon: pMB1 terminator: actin 8 |
| Applications： | promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--8.5; BamHI--8.5; BglII--8.5. A promoter-cloning plasmid vector using expression of beta-galactosidase as the reporter in Dictyostelium. pDdGal-15 (ATCC 37770), pDdGal-16 (ATCC 37771) and pDdGal-17 (TS153596) differ in their polylinker sequences. Each contains a BglII site in a different reading frame relative to the lacZ gene. The neo gene is regulated by an actin 6 promoter and a CP1 terminator. The lacZ gene and Dictyostelium actin 8 terminator from pDdlac-1 were inserted into a derivative of pB10TP2 from which the f1 origin and and a lacZ fragment had been removed. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Harwood AJ, Drury L. New vectors for expression of the E. coli lacZ gene in Dictyostelium. Nucleic Acids Res. 18: 4292, 1990. PubMed: 2198542 |
| Shipped： | freeze-dried |