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| 产品名称： | pLamB2(21/1) |
|---|---|
| 商品货号： | TS153636 |
| Designations： | pLamB2(21/1) |
| GenBank Number： | J02930 |
| Species： | Mus musculus, mouse |
| Depositors： | I Oberbaumer |
| Applications： | The 1.9 kb StuI or 1.7 kb HindIII/SspI fragments are recommended for hybridization. |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.517999887466431 Tissue: teratocarcinoma PYS-2/12 cell line Gene product: laminin, gamma 1 (formerly LAMB2)(laminin, B2 polypeptide) Lamb2 Alleles: a, b, d, d |
| Insert Size (kb)： | 2.518 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.6 (doublet); EcoRI--5.2; HindIII--3.4, 1.8; XbaI--3.6, 1.6; PstI--4.2, 1.0. The insert contains the following restriction sites (approximate kb from the 5 end): StuI--0.02, 1.91; HindIII--0.78; XbaI--1.02; PstI--1.68; SacI--1.99; SspI--2.5. Extends from nt 5036 to 7544 of the reported sequence, containing most of the 3 untranslated region. The 1.9 kb StuI or 1.7 kb HindIII/SspI fragments are recommended for hybridization. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975 Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992. Durkin ME, et al. The primary structure of the mouse laminin B2 chain and comparison with laminin B1. Biochemistry 27: 5198-5204, 1988. PubMed: 3167041 Ilse Oberbaumer, personal communication |