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| 产品名称： | pACHRBcDNA ACh receptor beta cDNA |
|---|---|
| 商品货号： | TS153650 |
| Designations： | pACHRBcDNA ACh receptor beta cDNA |
| GenBank Number： | M14537 |
| Species： | Mus musculus, mouse |
| Depositors： | JP Merlie |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.075000047683716 Tissue: muscle BC3H-1 cell line Gene product: cholinergic receptor, nicotinic, beta polypeptide 2( cholinergic receptor, nicotinic, beta subunit 2, acetylcholine receptor beta 2 neural) Acrb |
| Insert Size (kb)： | 2.075 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites (nt position according to published sequence): SacII--68; PvuII--450; PstI--455, 1538; BamHI--556; BglII--1044, 1858, 1918; BstXI--1399. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; BamHI--3.4, 1.7; HindIII--5.0; PstI--3.6, 1.1, 0.6; BglII--4.2, 0.9. Contains the complete coding sequence (nt 2-1507). Detects a mRNA of 2.4 kb. Oriented such that transcription from the SP6 promoter gives the sense strand. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Buonanno A, et al. A universal oliglonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit. J. Biol. Chem. 261: 16451-16458, 1986. PubMed: 3782129 Heidmann O, et al. Chromosomal localization of muscle nicotinic acetylcholine receptor genes in the mouse. Science 234: 866-868, 1986. PubMed: 3022377 John P Merlie, personal communication |