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| 产品名称： | p146-5 |
|---|---|
| 商品货号： | TS153692 |
| Designations： | p146-5 |
| Species： | Homo sapiens, human |
| Depositors： | HG Zachau, W Pargent |
| Vector： | Construct size (kb): 3.5 |
| Insert： | DNA: genomic Insert lengths(kb): 0.5 Tissue: placenta St Gene product: immunoglobulin kappa variable region IGKV Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp |
| Insert Size (kb)： | 0.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 0.5; PvuII--2.5, 0.6, 0.4; PvuI--2.4, 1.1; PvuI/EcoRI--1.7, 1.1, 0.7; PvuII/XbaI--2.5, 0.4, 0.3, 0.2, 0.2. Detects sequences from the Op (between O18 and A15) and Od (between O8 and O9) regions. In the AccI/BamHI digests of genomic DNA, the AccI site is polymorphic. Recloned from m146-5. The insert contains a PvuII site approximately 0.1 kb from the HindII end. |
| References： | Pargent W, et al. Polymorphisms and haplotypes in the human immunoglobulin kappa locus. Eur. J. Immunol. 21: 1829-1835, 1991. PubMed: 1678350 Pargent W, et al. The human immunoglobulin kappa locus. Characterization of the duplicated O regions. Eur. J. Immunol. 21: 1821-1827, 1991. PubMed: 1907917 Hans G Zachau, personal communication |