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| 产品名称： | pJK142 |
|---|---|
| 商品货号： | TS153835 |
| Designations： | pJK142 |
| Depositors： | JB Keeney |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.2720000743865970 Vector: pJK142 (plasmid) Promoters: Promoter lac Marker(s):ampR Construct size (kb): 3.272000074386597 Features: insert detection: lac marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | expression vector vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.3; EcoRI--3.3; HindIII--3.3. Expression vector permitting visual detection of recombinants by lacZalpha complementation. The order of the major features in the plasmid is: ampR - pMB1 ori - 5 lacZ - SacI/MCS/KpnI - 3 lacZ - NdeI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439 Jill B Keeney, personal communication |
| Shipped： | freeze-dried |