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| 产品名称： | pHE1 |
|---|---|
| 商品货号： | TS153849 |
| Designations： | pHE1 |
| Species： | Homo sapiens, human |
| Depositors： | GE Mark |
| Vector： | Construct size (kb): 6.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.799999952316284 Tissue: fetal liver Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1( Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1) RAF1 Alleles: B1, A1, A2, B2 |
| Insert Size (kb)： | 1.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | EcoRI linkers added to cDNA before insertion; insert oriented with HindIII site of insert closer to PstI site of pBR322. Restriction digests of the clone give the following sizes (kb): EcoRI--4.35, 1.8; BglII--6.2; HindIII--6.2. Insert contains HindIII, NdeI, BglII, EcoRII, TaqI, HincII, XbaI, ApaI, PvuII, and AccI sites. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References： | Gribbin TE, et al. Association of a mature B cell leukemia with a 4p+ chromosomal abnormality: derivation and characterization of a cell line. Leukemia 3: 643-647, 1989. PubMed: 2548046 Bonner TI, et al. Structure and biological activity of human homologs of the raf/mil oncogene. Mol. Cell. Biol. 5: 1400-1407, 1985. PubMed: 2993863 Barbara Weiffenbach, personal communication George E Mark, personal communication |