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微信小章| 产品名称： | A375-P |
|---|---|
| 商品货号： | TS153874 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | frozen 1.0 mL |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant melanoma |
| Age： | 54 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is useful as a control for A375-MA1 and A375-MA2 to study the mechanisms of metastasis. xa0It has been used with microarray analyses to identify metastasis-specific genes using a functional genomics approach and in proteomics analyses |
| Images： |  |
| Derivation： | This increased metastatic cell line was derived using an in vivo selection process of highly metastatic cells from a population of poorly metastatic tumor cells, A375 (ATCC® No. CRL-1619™) and A375P a donation from from Dr. Isaiah Fidler (Texas MD Anderson Cancer Center, for Set F cells).xa0 A375P and CRL-1619 were used to create more lung metastases cell lines when injected into mice.The A375-M1 (ATCC®xa0No. CRL-3222™) cell line was derived by i.v. injection of A375 cells into nude mice. Lung metastases were harvested and amplified in vitro as cell lines. The A375-M1 cell line was reinjected into mice for a second round of selection, lung metastases harvested and amplified in vitro as A375-M2 (ATCC® No. CRL-3223™) cells. The A375-M1 and A375-M2 cell lines were xa0transfected with a plasmid containing the ecotropic receptor for murine retrovirus and selected for neomycin resistance. This is useful for RNAse protection assays. |
| Tumorigenic： | yes |
| Effects： | Yes, in immunosuppressed mice |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0
Medium Renewal: Every 2 to 3 days |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Volume： | 1.0 mL |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 11,14 D16S539: 9 D5S818: 12 D7S820: 9 THO1: 8 TPOX: 8,10 vWA: 16,17 |
| Name of Depositor： | Richard Hynes, Ph.D. |
| Year of Origin： | 2004 |
| References： | Xu L, et al. Gene expression changes in an animal melanoma model correlate with aggressiveness of human melanoma metastases. Mol Cancer Res 6:760-769, 2008. PubMed: 18505921 Clark EA, et al. Genomic analysis of metastasis reveals an essential role for rhoC. Nature 406:532-535, 2000. PubMed: 1095231 |