| 产品名称: | Sp3 |
|---|---|
| 商品货号: | TS153877 |
| Designations: | Sp3 |
| GenBank Number: | M97191 |
| Species: | Homo sapiens, human |
| Applications: | contains sequence Sp3 transcription factor |
| Vector: | Construct size (kb): 4.900000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSP72 Intact vector size: 2.462 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI SacI EcoRI ClaI EcoRV BglII Polylinker sites: XhoI PvuII HindIII SphI PstI SalI AccI XbaI BamHI SmaI KpnI SacI EcoRI ClaI EcoRV BglII Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SP6, T7 replicon: pMB1 Cross references: DNA Seq. Acc.: X65332 |
| Insert: | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SP3 Gene name: Sp3 transcription factor Contains complete coding sequence?: Y Tissue: Hut78 cell line, PMA activated Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 2.4 Cross references: DNA Seq. Acc.: M97191 Nucleotides 1-2221 of the insert correspond to nucleotides 1-2221 of M97191. Insert lengths(kb): 2.400000095367432 Tissue: Hut78 cell line, PMA activated Gene product: Sp3 transcription factor SP3 Target Gene: Sp3 transcription factor |
| Insert Size (kb): | 2.400 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments: | Restriction digests of the clone give the following sizes (kb): BglII--3.8, 0.96; EcoRI--2.5, 2.2; NotI--4.7; PstI--3.0, 0.8, 0.6, 0.3; XbaI--4.7. Insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.89; EcoRI--0.0, 2.22; EcoRV--2.07; KpnI--1.63; NcoI--1.06; NotI--0.04; PstI--0.66, 1.43, 1.98; PvuII--1.12; SacI--1.71; XbaI--0.62. |
| References: | Kingsley C, Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol. Cell. Biol. 12: 4251-4261, 1992. PubMed: 1341900 |