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微信小章| 产品名称： | pCER204 in Escherichia coli |
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| 商品货号： | TS153922 |
| Designations： | pCER204 in Escherichia coli |
| GenBank Number： | X03363 |
| Species： | Homo sapiens, human |
| Depositors： | T Yamamoto |
| Vector： | Construct size (kb): 7.099
Name of vector: pcD Features (with orientation and position when available): |
| Insert： | DNA: cDNA
Insert Lengths (kb): 4.0
Tissue: gastric cancer MKN-7 cell line
Gene Product: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)( Avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2, Neu oncogene, avian erythroblastosis oncogene B 2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogen, tyrosine kinase, receptor HER2) NGL
Genome: Homo sapiens Gene Symbol: ERBB2 Other Gene Symbols: NGL Genomic Copy Number: unique Contains Complete Coding Sequence?: U Chromosome: 17; Localization: 17 q11.2-q12 Type of DNA: cDNA Insert 5 end:xa0xa0 Modification: GC Insert 3 end:xa0xa0 Modification: AT Insert size (kb): 4.0 Cross References: DNA Seq. Acc.: X03363xa0 |
| Insert Size (kb)： | 4.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--7.1; PvuII--3.0, 2.0, 0.9, 0.5, 0.4, 0.3; BamHI--4.9, 1.3, 0.7, 0.2; EcoRI--3.8, 1.8, 1.5.
Restriction digests of the clone give the following sizes (kb): HindIII--6.8; PvuII--2.95, 1.95, 0.85, 0.5, 0.4, 0.25; HindIII/PvuII--2.3, 1.95, 0.85, 0.54, 0.5, 0.4, 0.25; BamHI--4.7, 1.15, 0.6, 0.2; EcoRI--3.7, 1.65,xa0 1.35.
xa0 The insert is not readily excised from the vector (flanked by GC and AT tails). StuI cuts twice in the insert but not in the vector. The vector is cut with HindIII. A StuI/HindIII double digest gives a 3.4 kb fragment specific to c-erbB-2. Contains AccI, BamHI, EcoRI, SmaI, PvuII, and KpnI sites.
Includes sequence from approximately 500 to end in Fig. 2 encoding amino acids from about 100-1255 (including the ATP binding site of the tyrosine kinase domain). |
| References： | Zhang X, et al. Amplification and rearrangement of c-erb B proto-oncogenes in cancer of human female genital tract. Oncogene 4: 985-989, 1989. PubMed: 2569708 Yamamoto T, et al. Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor. Nature 319: 230-234, 1986. PubMed: 3003577 Fukushige S, et al. Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line. Mol. Cell. Biol. 6: 955-958, 1986. PubMed: 2430175 Ishii S, et al. Characterization of the promoter region of the human c-erbB-2 protooncogene. Proc. Natl. Acad. Sci. USA 84: 4374-4378, 1987. PubMed: 2885835 |