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| 产品名称： | HF hSNF2L |
|---|---|
| 商品货号： | TS153990 |
| Designations： | HF hSNF2L |
| GenBank Number： | M88163 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-3Zf(-) Intact vector size: 3.200 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: T7 - EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII - SP6 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SNF2L Genomic copy number: unique Gene name: SNF2 (sucrose nonfermenting, yeast, homolog)-like Contains complete coding sequence?: Y Chromosome: X; Localization: X q25 Tissue: fibroblast Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 3.6 Cross references: DNA Seq. Acc.: M88163 Insert lengths(kb): 3.599999904632568 Tissue: fibroblast Gene product: SNF2 (sucrose nonfermenting, yeast, homolog)-like SNF2L |
| Insert Size (kb)： | 3.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.6, 0.7, 0.3; HindIII--4.0, 1.4, 1.3, 0.2; PvuII--2.7 (doublet), 1.35; SacI--5.5, 0.9, 0.45; SalI--4.0, 2.2, 0.6. The insert contains the following restriction sites (bp from the 5 end): EcoRI--2681, 2938; HindIII--871, 2122, 3478; NcoI--2933; NdeI--434; PstI--998, 2006; PvuII--2603; SacI--2299, 3180; SalI--900, 1496; XhoI--2558. Contains the complete coding sequence (nt 142-3072). Sequence shows strong homology to the Saccharomyces cerevisiae transcription activator genes SNF2/SWI2 and STH1. The insert detects a 4.4 kb transcript in the following human tissues: heart, brain, placenta, lung, liver, skeletal muscle and kidney. |
| References： | Okabe I, et al. Cloning of human and bovine homologs of SNF2/SWI2: a global activator of transcription of yeast S. cerevisiae. Nucleic Acids Res. 20: 4649-4655, 1992. PubMed: 1408766 |