| 产品名称: | c9998 9998 |
|---|---|
| 商品货号: | TS154009 |
| Designations: | c9998 9998 |
| GenBank Number: | U00028 U00030 |
| Species: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors: | MV Olson, L Riles |
| Vector: | Construct size (kb): 41.70000076293945 |
| Insert: | DNA: genomic Insert lengths(kb): 35.28099822998047 Gene product: exopolyphosphatase ERG9 |
| Insert Size (kb): | 35.281 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products: | component of:ATCC 77284 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.511, 1.475, 4.032, 0.888, 0.457, 3.454, 8.355, 2.012, 5.306, 0.882, 0.413, 1.999, 0.976, 1.634, 1.517. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.86, 0.98, 1.5, 1.6, 2.0, 3.4, 4.0, 5.4, 8.5. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70098, 70403, 70799, 70886, 70890. Corresponds to nt 473207-508488 of the sequence of chromosome VIII. |
| Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References: | Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |