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| 产品名称： | Adeno-associated virus 3 |
|---|---|
| 商品货号： | TS154037 |
| Classification： | Parvoviridae, Dependovirus |
| Agent： | Adeno-associated virus 3 |
| Strain： | H |
| Common Name： | AAV-3H |
| Applications： | Cross-reacts with AAV-2; no hemagglutinin detected. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Requires co-infection with helper adenovirus Adenovirus type 2 (ATCC VR-846) for replication. Helper virus may be inactivated after harvest in water bath at 56°C for 30 minutes. Cross-reacts with AAV-2; no hemagglutinin detected. |
| Effect on Host： | AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing. |
| Recommended Host： | Production Host: HEK-293 (ATCC CRL-1573). Production Host: xa0HEK-293 (ATCC® CRL-1573x99) Alternate Hosts: HEK cells, KB cells (ATCC CCL-17) and HeLa cells (ATCC CCL-2) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with helper adenovirus and a small volume (e.g. 1 mL per 25 cm2) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 2-3 days at 37°C temperature in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture. |
| Name of Depositor： | MD Hoggan |
| Chain of Custody： | Deposited as Adeno-associated virus type 3 |
| Source： | Adenovirus 7, SV-40 hybrid E 46 plus strain infected HEK cells |
| References： | Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666 |