微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pJQ200 |
|---|---|
| 商品货号： | TS154094 |
| Designations： | pJQ200 |
| Depositors： | MF Hynes |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH10B |
| Vector Information： | Size (kb): 4.9000000953674320 DESCRIPTION OF VECTOR: Intact vector size: 4.900 Type of vector: plasmid Cloning sites: SmaI Polylinker sites: Construction: P15A ori, sacB, RP4, gtmR Host range: broad host range; Escherichia coli Features (with orientation and position when available): replicon: oriV, p15A other: traJ, marker(s): sacB, -> replicon: oriT marker(s): gtmR, Vector: pJQ200 (plasmid) Construction: P15A ori, sacB, RP4, gtmR Marker(s):sacB,gtmR Construct size (kb): 4.900000095367432 Features: marker(s): gtmR marker(s): sacB other: traJ replicon: oriT replicon: oriV, p15A |
| Applications： | integrating vector vector permitting construction of fusion proteins vector permitting positive selection for integration vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--5.2; EcoRI--5.2; HindIII--5.2. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g. as a BamHI cassette). When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. The order of the major features of the plasmid is: gtmR - SmaI - P15A - traJ - oriT - sacB. |
| Media： | ATCC® Medium 1885: LB medium (ATCC medium 1065) with 15 mcg/ml gentamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Quandt J, Hynes MF. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127: 15-21, 1993. PubMed: 8486283 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |