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产品简介
购买须知
| 产品名称： | pBCL8.1 |
|---|---|
| 商品货号： | TS154126 |
| Designations： | pBCL8.1 |
| Depositors： | CT Denny |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.1000003814697270 Vector: pBCL8.1 (plasmid) Construction: pBluescriptII KS+, pBP108, pBP103, LYS2 Marker(s):ampR,LYS2 Construct size (kb): 8.100000381469727 Features: marker(s): ampR, LYS2 replicon: pMB1 |
| Applications： | vector permitting positive selection for integration |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--8.2; PstI--8.2; XbaI--8.2. Recombinant deletion YACs can be recovered by digestion with BglII, EcoRI, HindIII, PstI, SacI or XbaI, recircularization, and transformation into E. coli. pBCL8.1 should be linearized with SalI before transformation into human YAC-containing yeast strains. Lys+ Trp+ Ura- colonies should be selected. Chromosome acentric fragmentation vector containing LYS2 for use with artificial chromosomes containing Alu repeats. The order of the major features in the plasmid is: LYS2 - HindIII - telomere - SalI - Alu repeats - pMB1 ori - ampR - SacI - XbaI - PstI - EcoRI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lewis BC, et al. Creation of a yeast artificial chromosome fragmentation vector based on lysine-2. Genet. Anal. Tech. Appl. 9: 86-90, 1992. PubMed: 1476795 |
| Shipped： | freeze-dried |