微信小陈
微信小章| 产品名称： | pcJE-1 |
|---|---|
| 商品货号： | TS154172 |
| Designations： | pcJE-1 |
| GenBank Number： | M19681 |
| Species： | Mus musculus, mouse |
| Depositors： | CD Stiles |
| Applications： | Frequently used as a molecular marker for response to cellular growth factors. Has been used to identify the human homolog from WI-38 cells. |
| Vector： | Construct size (kb): 3.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6000000238418579 Tissue: 3T3 (clone A31) cell line Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) Scya2 |
| Insert Size (kb)： | 0.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 0.63; BamHI--uncut; consistent with deposited DNA. Transcription from the SP6 promoter yields sense RNA. JE is one of several (including KC, JB) competence genes which are induced with the addition of platelet-derived growth factor. Frequently used as a molecular marker for response to cellular growth factors. Has been used to identify the human homolog from WI-38 cells. Includes a single open reading frame that presumably encodes a protein of 148 aa, with a consensus sequence for N-glycosylation at aa 126. The 3 region contains an ATTTA sequence described for cytokines and mRNAs with rapid turnover. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Cochran BH, et al. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33: 939-947, 1983. PubMed: 6872001 Rollins BJ, et al. Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties. Proc. Natl. Acad. Sci. USA 85: 3738-3742, 1988. PubMed: 3287374 Timmers HT, et al. E1A products of adenoviruses reduce the expression of cellular proliferation-associated genes. Oncogene Res. 3: 67-76, 1988. PubMed: 2974528 Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477 Wilson SD, Dorf ME. Localization of five cytokine genes to chromosome 11. Mouse News Lett. 85: 14-15, 1989. |