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| 产品名称： | pGC3 BB915 |
|---|---|
| 商品货号： | TS154196 |
| Designations： | pGC3 BB915 |
| Depositors： | JD Boeke |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.4609999656677250 Vector: pGC3 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS413 Marker(s):ampR,HIS3,MET15 Construct size (kb): 7.460999965667725 Features: insert detection: lacZ marker(s): HIS3 marker(s): MET15 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: BamHI...KpnI MCS: SacI...EagI centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.2; EcoRI--4.8, 1.4, 0.94; PvuII--4.4, 2.7. The insert contains the following restriction sites (approximate kb from the 5 end): XbaI--0.72; EcoRI--0.99, 2.37; HindIII--1.70; ScaI--1.96. An SpeI digest removes an intact 1.334 kb MET15 cassette, that includes flanking sequences of 577 bp at the 5 end and 580 bp at the 3 end). MET15, MET17 and MET25 are synonymous. The sequence of the PCR-generated MET15 cassette has not been determined to eliminate the possibility of PCR error. It was selected based on the described functional assay. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447 Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 |
| Shipped： | freeze-dried |