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| 产品名称： | pNKY48 |
|---|---|
| 商品货号： | TS154260 |
| Designations： | pNKY48 |
| Depositors： | N Kleckner |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.3000001907348630 Vector: pNKY48 (plasmid) Construction: pGC-1, pNKY2029 Marker(s):URA3,ampR Construct size (kb): 4.300000190734863 Features: marker(s): ampR, URA3 replicon: pMB1, M13 terminator: URA3 |
| Applications： | YI-type (integrating) shuttle vector contains easily purifiable cassette(s) for construction contains sequence histidinol dehydrogenase contains sequence uridine monophosphate synthetase UMP synthase, orotate phosphoribosyltransferase, orotidine 5-phosphate decarboxylase, orotate phosphoribosyltransferase 1 vector permitting construction of fusion proteins vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.4; BglII--4.4; HindIII--3.1, 1.3; EcoRI--4.4; XbaI--4.4. Contains a HIS4C/URA3 fusion cassette that can be used to make fusions to other proteins of interest. Also includes a M13 sequencing primer approximately 300 bp from the HIS4C/URA3 fusion junction. Encodes 93 amino acids of HIS4C and the complete 267 amino acids of URA3. Can be used for promoter/enhancer trap screens. Constructed by inserting a 1.4 kb HindIII fragment from pNKY2029 containing the HIS4/URA3 fusion into pNKY47, which is a derivative of pGC-1 with the original HindIII site destroyed and a new one created at the BamHI site of the MCS. The order of the major features in this plasmid is: M13 primer - ClaI - XhoI - SalI - XbaI (stop) - BamHI - HindIII - 90 aa HIS4C bridge/URA3 - BamHI - HindIII - BamHI - BglII - NcoI - EcoRI - M13 ori - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alani E, Kleckner N. A new type of fusion analysis applicable to many organisms: protein fusions to the URA3 gene of yeast. Genetics 117: 5-12, 1987. PubMed: 3311876 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 Nancy Kleckner, personal communication |
| Shipped： | freeze-dried |