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| 产品名称： | VMM425 |
|---|---|
| 商品货号： | TS154286 |
| Organism： | Homo sapiens, human |
| Tissue： | Melanoma, Pelvic Soft Tissue Metastasis |
| Cell Type： | Melanocyte |
| Product Format： | frozen |
| Morphology： | Epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Melanoma, Stage IV; malignant |
| Age： | 60 |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Applications： | Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Derived from a pelvic soft tissue recurrence. Established from digest of Right Pelvic Tumor Metastasis in PETI Medium. |
| Clinical Data： | Primary Site: Left Foot (Plantar); Metastatic Site: Pelvic Soft Tissue |
| HLA Typing： | A2,A31,B7,B51,Cw7?,Cw16?,DR4(DRB1*0404),DR11(DRB1*1101) or DR13(DRB1*131401),DR52(DRB3*0202),DR53(DRB4*0103) |
| Comments： | NRAS Mutation: Q61L Q61R CDKN2A: wt BRAF: wt PET Scan + |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes are for a T-75 flask; Adjust accordingly
|
| Cryopreservation： | Fetal bovine serum, 90%; DMSO, 10% |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% |
| STR Profile： | Amelogenin: X xa0 xa0xa0 D5S818: 11 D13S317: 9,13xa0 D7S820: 9 D16S539: 12 vWA: 17,19 TH01: 8,9.3 xa0 TPOX: 8,12 CSF1PO: 12 |
| Sterility Tests： | Pass |
| Population Doubling Level (PDL)： | unknown |
| Population Doubling Capacity： | unknown |
| Name of Depositor： | Craig L. Slingluff, Jr. M.D. |
| Passage History： | Original deposit at one passage from the primary culture |
| Year of Origin： | 2007 |
| References： | Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344 |