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| 产品名称： | pMON546 |
|---|---|
| 商品货号： | TS154305 |
| Designations： | pMON546 |
| Depositors： | Monsanto Company, DR Hoerner, Monsanto Company |
| Applications： | in suitable host, produces protein 5-enolpyruvylshikimate-3-phosphate synthase integration helper plasmid shuttle vector |
| Vector： | Construct size (kb): 180.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pMON530 Intact vector size: Type of vector: plasmid Vector end: BglII Vector end: EcoRI Cloning sites: BglII ClaI KpnI XhoI EcoRI Polylinker sites: BglII ClaI KpnI XhoI EcoRI Construction: pMON316 pMON526 Host range: Agrobacterium tumefaciens; Escherichia coli Features (with orientation and position when available): coding sequence: NOS, -> marker(s): spcR marker(s): strR marker(s): kanR, terminator: NOS polyadenylation signal, MCS: EcoRI...BglII, -> promoter: CaMV, replicon: oriT replicon: pMB1 Cross references: |
| Insert： | Gene product: 5-enolpyruvylshikimate-3-phosphate synthase EPSPS DESCRIPTION OF INSERT COMPONENT: Genome: Petunia sp. Gene symbol: EPSPS Gene name: 5-enolpyruvylshikimate-3-phosphate synthase Contains complete coding sequence?: Y Tissue: MG4-G cell line Type of DNA: cDNA Insert end: EcoRI Modification: EcoRI -> BglII Insert end: EcoRI Insert size (kb): 1.95 Cross references: Target Gene: , 5-enolpyruvylshikimate-3-phosphate synthase |
| Media： | 1236 + spectinomycin(50 mcg/ml) + streptomycin(50 mcg/ml) + chloramphenicol(25 mcg/ml)
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | A BglII+EcoRI digest produces two fragments from the insert. The approx. 330 bp BglII/EcoRI fragment contains 5 non-coding region and sequences encoding a chloroplast transit peptide and the 5 end of EPSPS. The 1.62 kb EcoRI fragment encodes the 3 end of EPSPS and contains the poly(A) tail. pMON546 contains a nopaline synthase/neomycin phosphotransferase II fusion gene that confers kanR only in transformed plant tissue. Both pMON546 and pGV3111SE can be isolated from the A. tumefaciens culture by co-culturing with E. coli containing a helper mobilization plasmid like pRK2013. E. coli with pMON546 are spcR strR kanS, while E. coli with pGV311SE are spcS strS kanR. pGV3111SE is a crippled Ti plasmid (the region containing tms tmr tml and OCS has been deleted) that contains vir genes which will integrate T-DNA sequences into a host chromosome. When Petunia cells are transformed with A. tumifaciens containing pMON546 and pGV3111SE, the CaMV/EPSPS gene permits growth in the presence of 0.5mM glyphosate. Phenotype verified: kanR, spcR, strR, cmlR. |
| References： | Shah DM, et al. Glyphosate-resistant plants. US Patent 4,940,835 dated Jul 10 1990 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |