| 产品名称: | 8AC |
|---|---|
| 商品货号: | TS154310 |
| Designations: | 8AC |
| GenBank Number: | M60493 |
| Species: | Mus musculus, mouse |
| Depositors: | T Yorifuji, AL Beaudet |
| Vector: | Construct size (kb): 6.820000171661377 |
| Insert: | DNA: cDNA Insert lengths(kb): 3.880000114440918 Gene product: cystic fibrosis transmembrane conductance regulator Cftr |
| Insert Size (kb): | 3.880 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 1.5, 0.65; EcoRI--5.4, 1.45; HindIII--3.2, 2.3, 0.85, 0.7; PstI--6.9; PstI/XhoI--3.9, 2.9. The insert contains the following restriction sites (nt from the 5 end): HindIII--85, 856, 1539, 3792; SalI--568; BamHI--1600, 2252; EcoRI--1510. The insert extends from nt 2438-6304 of the reported sequence, including exons 13 through the 3 end. Also includes a short (14 nt) poly(A) tail not in the sequence record. Transcription from the T3 promoter gives the sense strand. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Oliva D, et al. Complete structure of the human gene encoding neuron-specific enolase. Genomics 10: 157-165, 1991. PubMed: 2045099 Tohru Yorifuji, personal communication |