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| 产品名称： | DM11 |
|---|---|
| 商品货号： | TS154370 |
| Designations： | DM11 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 45.90000152587891 |
| Insert： | DNA: genomic Insert lengths(kb): 15.19999980926514 Gene product: DNA Segment, single copy D15S74 Alleles: A1, A2, B1, C1, C2, B2 |
| Insert Size (kb)： | 15.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--19.0, 11.0 (doublet), 4.2; BamHI--23.0, 7.4, 5.8, 4.0, 2.5 1.5; HindIII--23.0, 9.4, 5.8, 4.0, 3.3, 0.96; PstI--14.0, 9.6, 5.3, 3.0, 2.7 (doublet), 2.2, 1.8, 1.2, 0.86, 0.5; AvaI--19.0, 10.0, 6.6, 4.6, 3.1, 2.4, 1.6, 1.2. The allele frequencies of the MspI B1/B2 and the StuI C1/C2 polymorphisms show significant population dependence. The MspI A1/A2 alleles can be detected with a 3.3 kb MspI fragment from the insert. The MspI B1/B2 alleles can be detected with the 5.5 or 5.1 kb MspI fragments from the insert. This clone was isolated from a HaeIII/AluI genomic library in which the DNA was ligated to EcoRI linkers. The sizes of the EcoRI fragments in the insert are (kb): 11.0 and 4.2. Enzyme(s) not detecting polymorphism: AvaII, BamHI, BglII, EcoRI, HincII, HindIII, MboI, PstI, PvuII, RsaI, SstI, TaqI (tested 7 individuals). |
| References： | Morris DJ, et al. A new marker on chromosome 15, D15S74, detects three RFLPs. Nucleic Acids Res. 17: 7121, 1989. PubMed: 2476728 Michele Ramsay, personal communication |