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| 产品名称： | pAD1 |
|---|---|
| 商品货号： | TS154426 |
| Designations： | pAD1 |
| Depositors： | JD Boeke |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli unknown (ATCC 33694) |
| Vector Information： | Size (kb): 6.485 DESCRIPTION OF VECTOR: Intact vector size: 6.485 Type of vector: phagemid Cloning sites: Polylinker sites: Other unique sites: BamHI Construction: pRS406 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): URA3, -> replicon: f1, other: LEU2 deleter cassette replicon: pMB1 marker(s): ampR, Vector: pAD1 (phagemid) Construction: pRS406 Marker(s):URA3,ampR Construct size (kb): 6.485 Features: marker(s): URA3 marker(s): ampR other: LEU2 deleter cassette replicon: f1 replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector marker deletion vector 3-isopropylmalate dehydrogenase beta-isopropylmalate dehydrogenase |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--6.5; KpnI/SstI--4.3, 2.2; ClaI--6.5. The two step selection process requires a ura3 transformation host (this host can be created using pJL164 (ATCC 87471)). After transformation with the SalI linearized vector, URA3 integrants are selected on ura- plates. The designer deletion strain is then recovered by selection on 5-FOA plates (loss of URA3 and LEU2 markers by a homologous recombination event). This deleter vector is used to create designer yeast strains with a non-revertable leu2 auxotrophic marker deletion. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Related Products： | component of:ATCC 87472 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |