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微信小章| 产品名称： | TPO3 |
|---|---|
| 商品货号： | TS154498 |
| Designations： | TPO3 |
| GenBank Number： | Y00406 |
| Species： | Homo sapiens, human |
| Applications： | Codominant segregation observed in 9 (PstI RFLP) and 2 (BglII RFLP) families (38 and 10 individuals, respectively). |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.0 Tissue: thyroid Gene product: thyroid peroxidase TPO Alleles: A2, C1, A1, B1, B2, C2, A1, A2, A3, A4, A5, A6 |
| Insert Size (kb)： | 3.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Insert contains the following sites separated by (bp): (EcoRI)- 140- ClaI- 263- BglI- 469- BcnI- 142- MboII- 301- MspI- 72- ClaI- 20- AvaII- 950- RsaI- 198- AccI- 455- (EcoRI). Restriction digests of the clone give the following sizes (kb): XhoI--6.0; BglI--3.5, 1.5, 1.0; HindIII--6.0; EcoRI--3.0, 3.0; AccI--5.4, 0.5. Codominant segregation observed in 9 (PstI RFLP) and 2 (BglII RFLP) families (38 and 10 individuals, respectively). Insert contains most of the sequence (from position 77 to the poly(A) signal) of human thyroid peroxidase. DNA sequence shows 68% homology with the partial porcine thyroid peroxidase sequence. Also detects a VNTR polymorphism best visualized in BglII and EcoRI digests. Requires a last stringent wash (0.1X SSC, 65C). |
| References： | Libert F, et al. Complete nucleotide sequence of the human thyroperoxidase-microsomal antigen cDNA. Nucleic Acids Res. 15: 6735, 1987. PubMed: 3453124 Massaro G, et al. RFLPs detected at 2 pter-p12 with a thyroid peroxidase cDNA probe, TPO3 (McKusick No. 27450). Nucleic Acids Res. 17: 2155, 1989. PubMed: 2564671 Libert F, et al. Thyroperoxidase, an auto-antigen with a mosaic structure made of nuclear and mitochondrial gene modules. EMBO J. 6: 4193-4196, 1987. PubMed: 3443105 Gilbert Vassart, personal communication |