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微信小章| 产品名称： | Saccharomyces cerevisiae CEN BANK genomic library in E. coli |
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| 商品货号： | TS154539 |
| Designations： | Saccharomyces cerevisiae CEN BANK genomic library in E. coli |
| GenBank Definition： | Saccharomyces cerevisiae succinate dehydrogenase (SDHA) gene, Yeast DNA for CTP: phosphoethanolamine cytidylyltransferase and Saccharomyces cerevisiae succinate dehydrogenase membrane anchor Saccharomyces cerevisiae synaptobrevin (SNC2) gene, complete cds. |
| GenBank Number： | M86746 D50644 L26333 L16243 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | MD Rose |
| Vector： | DNA: Genomic
Digest: Partial
Insert size range: 10 - 20 kb
Genome Equivalent: 0.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen aliquots of the library in E. coli |
| Comments： | The vial labeled 95-02A corresponds to Cen Bank A and the vial labeled 95-02B corresponds to Cen Bank B.
Resuspend in a small amount of broth and plate for single colonies to avoid fast growing clones taking over the culture.
For Cen Bank A, the insert range is 10-15 kb, the number of clones for coverage is 8900, and number of independent recombinants is 8.9x103. There are 10% non-recombinants.
For Cen Bank B, the insert range is 15-20 kb, the number of clones for coverage is 9500, and the number of independent recombinants is 9.5x103. There are 10% non-recombinants.
The titer of Cen Bank A is about 1.5 x 109 cfu/mL and the titer of Cen Bank B is about 2.0 x 108 cfu/mL. The library has been amplified once by the ATCC.
GRF88 is an S288C derivative (MATalpha his4-38). |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Thrash C, et al. Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc. Natl. Acad. Sci. USA 82: 4374-4378, 1985. PubMed: 2989818 Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750 |