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| 产品名称： | pSLF101 |
|---|---|
| 商品货号： | TS154583 |
| Designations： | pSLF101 |
| Depositors： | SL Forsburg |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.8000001907348630 DESCRIPTION OF VECTOR: Intact vector size: 6.800 Type of vector: phagemid Cloning sites: SphI PstI SalI BamHI SmaI KpnI HpaI_ Polylinker sites: SphI PstI SalI BamHI SmaI KpnI HpaI Other unique sites: ScaI SspI Host range: Schizosaccharomyces pombe; Escherichia coli Features (with orientation and position when available): replicon: ars1 replicon: pMB1 marker(s): LEU2 operator: tet, promoter: CaMV, marker(s): ampR, Vector: pSLF101 (phagemid) Promoters: Promoter CaMV Marker(s):LEU2,ampR Construct size (kb): 6.800000190734863 Features: marker(s): LEU2 marker(s): ampR operator: tet promoter: CaMV replicon: ars1 replicon: pMB1 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.6, 2.2; BamHI--6.8; EcoRI--3.9, 1.2. The vector contains the constitutive CaMV promoter adjacent to the tet operator. The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+. A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437) has a tet repressor gene and integrating vector pSLF104 (ATCC 87621) contains the sup3-5 marker and tet repressor under adh promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516 |
| Related Products： | component of:ATCC 87685 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |