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| 产品名称： | Influenza A virus (H1N1) |
|---|---|
| 商品货号： | TS154585 |
| Classification： | Orthomyxoviridae, Influenzavirus A |
| Agent： | Influenza A virus (H1N1) |
| Strain： | A/Swine/Iowa/15/30 (TC-adapted) |
| Applications： | Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Derived by adaptation of egg-passage A/Swine/Iowa/15/30 (ATCC VR-333) to MDCK (ATCC CCL-34) cells. (Note: ATCC VR-333 and TS154585 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture). |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Derived by adaptation of egg-passage A/Swine/Iowa/15/30 (ATCC VR-333) to MDCK (ATCC CCL-34) cells. (Note: ATCC VR-333 and TS154585 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture). |
| Effect on Host： | CPE (cell rounding, degeneration, sloughing) CPE (cell rounding, degeneration, sloughing) |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) |
| Recommended Host： | Production Host: MDCK (ATCC CCL-34) Alternate Hosts: Chicken embryo, MDCK and Monkey kidney cells (MkK) Recommended Host:xa0 MDCK (ATCC® CCL-34x99) |
| Growth Conditions： | For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.5°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, TS154585 should be grown in serum free media that contains 1 ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock). |
| Name of Depositor： | A. P. Kendal and ATCC |
| Source： | Swine in Iowa, 1930 |
| References： | Shope RE. Swine influenza : i. experimental transmission and pathology. J. Exp. Med. 54: 349-359, 1931. PubMed: 19869922 |