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| 产品名称： | pTFE3-1.9 |
|---|---|
| 商品货号： | TS154589 |
| Designations： | pTFE3-1.9 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.78000020980835 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.815000057220459 Tissue: B lymphocyte Gene product: transcription factor binding to IGHM enhancer 3(transcription factor for IgH enhancer) TFE3 Alleles: A1, A2 |
| Insert Size (kb)： | 1.815 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert includes the following restriction sites (position according to the published sequence): PstI--677, 1859; NcoI--687, 2447; StuI--1111; HindIII--2216. Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 1.8; HindIII--4.5, 0.32; SmaI--4.8; PstI--3.8, 1.2; XhoI--4.8. The insert extends from a site 22 base pairs 5 to the PstI site at nucleotide 677 of the published sequence through the 3 end. It encodes amino acids 220 through 536 and includes the 3 untranslated region. Enzyme(s) not detecting polymorphism: BglI, BglII, DraI, EcoRI, MspI, PstI (20 unrelated X chromosomes), and TaqI (10 unrelated X chromosomes). |
| References： | Beckmann H, et al. TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif. Genes Dev. 4: 167-179, 1990. PubMed: 2338243 Puck JM, et al. A high-frequency RFLP at the human TFE3 locus on the X chromosome. Nucleic Acids Res. 19: 684, 1991. PubMed: 1672758 Paula S Henthorn, personal communication |