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| 产品名称： | Human rhinovirus 57 |
|---|---|
| 商品货号： | TS154743 |
| Classification： | Picornaviridae, Rhinovirus, Human rhinovirus A |
| Agent： | Human rhinovirus 57 |
| Strain： | Ch47 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Virus growth is optimal at pH 6.8-7.3, in roller or rocker cultures incubated at approximately 33°C. All members of the rhinovirus group are ether resistant. |
| Effect on Host： | Cytopathic effects (CPE) in culture include rounding and sloughing of cells xa0CPE in culture include cell rounding and sloughing |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host - H1-HeLa cells (ATCC CRL-1958)
Recommended Host: xa0H1 HeLa (ATCC® CRL-1958x99) Alternate Host(s) - WI-38 cells (ATCC CCL-75); MRC-5 cells (ATCC CCL-171); HeLa cells (ATCC CCL-2); other human cells |
| Growth Conditions： | Host - H1 HeLa cells (ATCC CRL-1958)
Medium - EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020) Infection conditions - For best results, plate cells 24-48 hours in advance. Infect at about 80% confluence using a multiplicity of infection (MOI) of 0.1 to 1.0. Adsorb virus to cells in a small volume for one hour. After adsorbtion, feed with sufficient medium to maintain cells and incubate for 1 to 7 days at 33 °C, with rocking, in a humidified atmosphere with 5% CO2 . Harvest culture fluid or fluid and cells when entire culture displays cytopathic effects. Preservation - Freeze as quickly as possible and store at -70°C or colder. |
| Name of Depositor： | NIAID, ATCC |
| Special Collection： | ATCC |
| Source： | Derived by passage of NIAID reagent V-112-001-021 (ATCC VR-1167) at ATCC |
| References： | NIAID Catalog of Research Reagents 1978-1980. S. Cunningham, ed. US Department of Health Education and Welfare, Public Health Service, National Institutes of Health; DHEW Pulication No. (NIH) 78-899, Bethesda, MD. |