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| 产品名称： | alpha s-Q227L (GSL) rat |
|---|---|
| 商品货号： | TS154843 |
| Designations： | alpha s-Q227L (GSL) rat |
| GenBank Number： | M12673 |
| Species： | Rattus norvegicus, rat |
| Depositors： | HR Bourne, P Herzmark |
| Applications： | in another host, produces protein guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.600000023841858 Gene product: guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 GNAS1 Target Gene: guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 |
| Insert Size (kb)： | 1.600 |
| Media： | ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 0.6; EcoRI--5.6; HindIII--5.6; HindIII/SalI--4.0, 1.6; SalI--5.6. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.4, 0.95; BglII--1.1; EcoRI--0.65; NcoI--0.2; PstI--0.3, 1.0; PvuII--0.3, 0.75. Deposited with the understanding that the DNAs not be used in human experiments. The protein produced is the constituitively activated Q227L mutation (glutamine replaced by leucine at codon 227). Encodes a hemagglutinin epitope replacing nt 475-489 of the GenBank sequence, followed by an altered serine codon (TCC) nt 490-492. Restriction site(s) at the 3 end of the insert for linearizing the construct: XhoI, NotI, HindIII. |
| References： | Levis MJ, Bourne HR. Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity. J. Cell Biol. 119: 1297-1307, 1992. PubMed: 1280272 Paul Herzmark, personal communication |